Plasmid_Backbone

Part:BBa_K5194007:Design

Designed by: Nirmal Cheema   Group: iGEM24_UCalgary   (2024-09-26)


pET 28a - 20061 plasmid vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 5138
    Illegal XhoI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5204
    Illegal NgoMIV site found at 315
    Illegal NgoMIV site found at 3358
    Illegal NgoMIV site found at 3518
    Illegal NgoMIV site found at 5106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2440


Design Notes

The plasmid was designed by Stephen Sligar.


Source

pMSP1D1 was a gift from Stephen Sligar (Addgene plasmid # 20061 ; http://n2t.net/addgene:20061 ; RRID:Addgene_20061) https://www.addgene.org/20061/

References